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Immunohistochemical staining (IHC) A HCC tissue microarray containing 10 pairs of tumor tissues and matched adjacent tissues (HLivH020PG01) and a PCa tissue microarray containing 3 normal tissues, 8 adjacent tissues and 49 tumor tissues (HProA060PG01) were purchased from Outdo Biotech (Shanghai, China)

3.2 Ultrasound-assisted extraction (UAE) Results concerning BoxBehnken (BB) experimental design for the second step (UAE) of the sequential process are presented in Supplementary Table S3

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