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Western blot assay Cells (1 10 6 ) were grown to 7080% confluency and treated with DMSO control, (1S,3R)-RSL3 (RSL3, Catalog # 19,288, Cayman Chemical, MI) or Fer1 (Medchemexpress, NJ) as per the experiments

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We tested the sensitivity of these erastin-resistant cell lines to the other types of ferroptosis inducers, RSL3 and FIN56, 30 which induce ferroptosis by inhibiting the glutathione peroxidase GPX4, a downstream antioxidant enzyme of system X c