For musculoskeletal, tendon, ligament, or systemic targets, injection is the better-supported route because all relevant animal healing studies used injected BPC-157, and systemic absorption from capsules in humans is unverified
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ELISA testing involved homogenizing a fraction of the tissues in a lysis solution supplemented with protease and phosphatase inhibitors (200 mM NaCl, 5 mM EDTA, 10 mM Tris, 10% glycerol