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Neuromics, MO15013), anti-GPX1 (1:500 dilution, GeneTex, GTX116040), anti-C-caspase 3 (1:400 dilution, Cell Signaling Technology, 9664), anti-NeuN (1:500, Cell Signaling Technology,12943), anti-4-HNE (1:500 dilution, Abcam, ab48506, United States), or anti-NF-B p65 (1:500 dilution, Cell Signaling Technology, 6956) diluted in the blocking solution at 4C overnight

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The identification of the mechanisms that contribute to or mitigate copper toxicity in WD is highly important

When administered by an experienced and medically trained practitioner the procedure is very safe with complications being extremely rare