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This effect requires GLP-1R expression in brain neurons, as shown in mice lacking GLP-1R in neuron-targeting Wnt1- or nestin-expressing domains ( Glp1r Wnt1/ and Glp1r Nes/ ), and by intracerebroventricular injection of exendin-9-39, a GLP-1R antagonist that suppresses activation of brain GLP-1R signaling

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Both nutrients exert antidepressant effects through gut microbiota regulation, either by enhancing SCFA production or reducing inflammation, making them the most promising candidates for clinical translation (consistent with Sections 4.3, 5.3)