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40 g of protein lysates, unless otherwise indicated, were resuspended in Laemmli buffer (20% glycerol, 125 mM Tris-HCl, 4% sodium dodecyl sulfate, 10% -mercaptoethanol, 0.075% bromophenol blue) 131 , separated in a 12% SDS-polyacrylamide gel electrophoreses (SDS-PAGE), and electroblotted to nitrocellulose

Bromelain has been extensively studied for its implication in cell death, indeed it can intervene in the expression of Bcl-2, a type of protein with an anti-apoptotic effect but can also positively regulates the expression of apoptotic genes, such as Bax, Apaf-1, and caspases-9 and -3 (87)

If a vial accidentally freezes, it must be discarded

Dineen R, Stewart PM, Sherlock M