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These second messengers, particularly cAMP, may activate protein kinases, enzymes considered responsible for phosphorylating target proteins
The most commonly studied neurometabolites are tNAA ( N -Acetyl Aspartate + N -Acetyl Aspartyl Glutamate), tCr (Creatine + Phosphocreatine), tCho (Choline + Phosphocholine + Glycero-phosphocholine), Glx (Glutamate + Glutamine), and mI (Inositol + Glycine)
The cell viability was assessed by Hoechst staining of the nucleus (see Methods) and hence assessment of total cell numbers after 72 h of treatment, which is a good proxy for cell survival and cell growth upon prolonged compound exposure