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To determine the effects of Exendin-4 (a GLP-1R agonist) and Compound C (an AMPK inhibitor, 6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine) on the localisation of K ATP channels in ischaemia, mice were subjected to 40 min of LAD ligation followed by 10 min of reperfusion, with Compound C (Dorsomorphin 2HCl, APExBIO

RNA quality was assessed by 1% agarose gel electrophoresis, and concentration was determined using a NanoDrop spectrophotometer

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