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Samples were processed for protein isolation using RIPA buffer (Boston Bio-product, Ashland, MA) with protease inhibitors cocktail (Cell signaling technology, Danvers, MA), quantified by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) and 40 g of proteins were boiled with 4 denaturing Laemmli Sample Buffer (Bio-Rad Laboratories, Hercules, CA) and loaded on 7.5% SDS-PAGE gel

Apart from binding to ECM ligands, integrins can also interact with other non-conventional extracellular ligands such as adhesion molecules (e.g., ICAM, VCAM), phospholipase A2, and fractalkine 35,36,48

& Marchetti, P

If irritation persists across multiple rotated sites, discontinue use and consult a healthcare provider