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Medium components before inoculum preparation and fermentation processes have to be sterilized separately by several groups (carbon sources, nitrogen sources, salts in water, and amino acids) to avoid Maillard reactions, in which products can become inhibitors for riboflavin production (Schwechheimer et al., 2016)

Moreover, in the presence of a further aldehyde or 2-ketoacid molecule acting as an acceptor, the C-2a-carbanion/enamine yields 2-hydroxyketones or hydroxyketoacids [31]