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Then add 500 mg of ALCAR

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In addition, drug repurposing may be particularly appropriate in conditions with high oxidative stress and comorbidities [110, 111]

After incubating for 30 min, excess metal ions were removed by buffer exchanging three times with buffer A, followed by two exchanges with 100 mM sodium phosphate buffer (pH 7.4) containing 1 mM cysteine