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The suspension was then ultracentrifuged for another hour at 41,000 rpm at 4C to separate detergent-resistant (DRM) from detergent-soluble (DSM) membrane fractions: the disordered DSM fraction in the supernatant was retained for analysis and the ordered DRM (lipid raft) fraction was resuspended in 1% SDS plus protease and phosphatase inhibitor cocktails, sonicated, centrifuged for 10 minutes at 13,500 rpm at 4C, and analysed via SDS-PAGE and Western blotting as in (25)