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Description

Published literature has examined IGF-1 LR3 across a range of cell-culture and animal research models relating to IGF-1R signal transduction biology, making it a frequently used research tool in IGF-1R-expressing cell lines

Proteins were digested after desalting (Amicon Ultra 3 kDa MWCO, Millipore), then subjected to heat denaturation (80 C), reduction, alkylation, and trypsin digestion (1:20 ratio trypsin:protein (w/w))