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The concentration of ethanol solution varied with the modeling time, increasing from 520% (mice were offered with 5% ethanol (v/v) for 1 week, then 10% ethanol (v/v) for the next 2 weeks, and 15% ethanol (v/v) for the next 3 weeks, finally animals had access to 20% ethanol (v/v) for last 4 weeks)
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Moreover, Mendelian randomization analyses support a causal role for specific gut microbes: Actinobacteria and Bifidobacterium taxa appear protective, while Lactobacillaceae increase anxiety risk (Lai and Xiong, 2025)
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