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Each extraction buffer contained the following protease inhibitors: 1 mM 1,10-phenanthroline, 1 mM phenylmethanesulfonyl fluoride, 334 M 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, 14 M E-64, 5.25 M leupeptin, 3 M bestatin, and 1 M pepstatin A