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The membranes were incubated with specific primary antibodies against 4-HNE (#ab46545, Abcam, USA), TFR (P02786, Abmart, China), FTH1 (sc-376594, Santa Cruz, USA), SP-C (#ab211326, Abcam, USA), GPX4 (#ab125066, Abcam, USA), ACSL4 (sc-365230, Santa Cruz, USA) and -actin (Cat # RM2001, Beijing Ray Antibody Biotech, China) overnight at 4 C

If you notice these changes after potential heat exposure, do not assume the dose needs increasing

Here's how it works: Remove the plastic caps from both the peptide vial and your bacteriostatic water