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It was also shown that TMA and TMAO levels were initially higher and the numbers of aortic lesions were increased in choline diet-fed ApoE / mice transplanted with microbiota from high TMAO-producing C57BL/6J strains than in mice transplanted with low TMAO-producing NZW/LacJ strains ( The final step in TMAO formation is the oxidation of TMA, which is mediated by the flavin-containing monooxygenase (FMO) family members FMO1 and FMO3