After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions
Unlike full-length human growth hormone, none of them seem to have any effect on blood sugar levels, insulin sensitivity or other hormonal pathways, this lessens the risk of major metabolic side effects
Epithalon, in this framework, is studied as a molecular signal that may influence how well those bindings are preserved during replication
Brain sections were blocked in 5% goat serum before overnight incubation of specific antibodies against albumin