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Loren Pickart estimated that toxic effects would begin at approximately 22,500 mg, which represents a 300-fold margin above effective therapeutic doses

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The choice of diluent depends on the study: sterile water is common for single-session work, while bacteriostatic water, which contains a small amount of benzyl alcohol, is often preferred when a solution will be drawn from over several days

Although such strategies have exhibited favorable outcomes in rheumatoid arthritis, their efficacy in OA remains debated, necessitating additional clinical trials (166, 167, 175)