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siRNA targeting LRP5 (GGACAGACUCAGAGAGACCAA) was transferred into the BMSCs with the help of Lipofectamine 3000 (Invitrogen, United States), and all operations were carried out under the manufacturers instructions
Research Highlights Metabolic Regulation and Anti-Obesity Effects: Potent inhibition of nicotinamide N-methyltransferase (NNMT) increases cellular NAD and S-adenosylmethionine (SAM) levels, enhances polyamine flux, boosts energy expenditure, and suppresses lipogenesis
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