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To directly validate the proposed roles of Vit A and Lup in sperm cryopreservation, future work could employ western blot analysis to quantify the expression and activation states of key proteins, such as NRF2 and Caspase 3, along with their downstream effectors, in sperm following freezethaw

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Antioxidant and anti-inflammatory properties of curcumin, in The molecular targets and therapeutic uses of curcumin in health and disease,

NAD + and NADH measurements (enzymatic assay) The frozen liver tissue (approximately 50 mg) was lysed in 500 l of ice-cold 0.6 M perchloric acid with a metal bead in the TissueLyser (Qiagen), with a frequency of 20 Hz for 2 min