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These alterations in the tumor ECM induce an increase in its stiffness, typically evaluated using techniques that either require direct contact with the sample (e.g., atomic force microscopy), or measure the tumor mechanical properties in a contact-free manner (e.g., shear wave elastography, magnetic resonance elastography) (Table 3)

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Animal study The C57BL/6 mice (male, 56 weeks old) were purchased from SPF (Beijing) Biotechnology Co., Ltd

In other embodiments, ultrasonic devices, flow through homogenizers or supercritical CO2 devices are used to prepare nanoparticles