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Resulting intact mitochondria were resuspended in Elution Buffer containing 250 mM sucrose, 0.02 mM EGTA, 10 mM Tris pH 7.4, 2 mM Tris-phosphate pH 7.4, and 5 mM MgCl 2

Is oxidative stress evaluated in viable human spermatozoa a marker of good semen quality

Oxidant resistance measurement We investigated whether FreSHtracer-isolated hES-MSCs have enhanced resistance to oxidative stress by treating them with diamide, an oxidizing agent that specifically oxidizes thiol groups at indicated concentrations (125, 250 and 500 M)