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Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research

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doi: 10.1152/ajpgi.00242.2016 13 CamilleriM.ShinA.BusciglioI.CarlsonP.AcostaA.BharuchaA

with 100 g TNP-Ficoll FITC (Biosearch Technologies F-1300F) and 30 min later tissues were analyzed with flow cytometry (splenocytes were stained with surface markers for the detection of FoB and MZB, with the addition of biotinylated anti-TNP (BD Pharmingen 554055) + Streptavidin-APC (Biolegend 405207 for TNP detection) or snap-frozen for immunofluorescence microscopy in OCT (Sakura 4583)