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Absorbance of culture media alone was considered as baseline

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Statistical analysis Assuming a proportion of participants experiencing overcorrection based on previous literature 13,16,21 , we determined that a sample size of 66 participants would yield 80% statistical power to detect a significant difference in the incidence of overcorrection between the proactive and reactive groups, employing an alpha () level of 0.05, beta () of 0.2, and accounting for a 10% dropout rate

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