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The KAR-binding HTLs (ShHTL1, ShHTL3, and AtHTL) had smaller pockets than the D14 group and ShHTLs of the divergent clade due to more bulky and hydrophobic residues

(3) Add 300 L of chloroform, vortex for 30 s, ultrasonic extraction for 10 min, leave at -20C for 20 min, centrifuge for 10 min (12000 rpm)

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They typically maintain temperatures around 64 to 72 degrees Fahrenheit, which is below the 86 degree danger threshold but well above actual refrigeration