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Establishment of 143B subcutaneous osteosarcoma mouse model and therapeutic effect evaluation Tumor model establishment and biodistribution study of NP4 in vivo Three female BALB/c nude mice were subcutaneously inoculated with 143B human osteosarcoma cells (3 10 6 ) on the right buttock

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Sterile syringes and needles with appropriate gauge and length based on the injection site