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The following primary antibodies were obtained from Cell Signaling Technology and used at a dilution of 1:1000: anti-Thioredoxin 1 (C63C6) (#2429), anti-TXNIP (D5F3E) (#14715), anti-Thioredoxin reductase 1 (TXNRD1) (#15140) and anti-GAPDH (14C10) (#2118)

Oxidative stress: antagonistic signaling for acclimation or cell death

Each patient receives a customized titration schedule: most users begin at a low dose and increase gradually over weeks to minimize gastrointestinal side effects while maximizing weight loss

Since synthetic mimics may independently act on peroxides, their efficacy may not be limited due to the complex processes involved in the biosynthesis of seleno-GPxs