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doi: 10.3389/fped.2014.00066 Received 09 May 2014 Accepted 09 June 2014 Published 27 June 2014 Volume 2 - 2014 Edited by Roberto Canitano, University Hospital of Siena, Italy Reviewed by Andrew Walter Zimmerman, Harvard Medical School, USA

10 Major risk factors for appendicitis include family history and male sex category

Total protein extracts were prepared by standard procedures and analyzed by PAGE using the following antibodies: caspase 3 active form, iNOS (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and Ub (Sigma-Aldrich)

If it is too thick, add a splash of milk