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In the present study, we have explored in depth this important region of these receptors both in vitro and in vivo by generating cell lines in which we have deleted both endogenous IR and IGF1R and then reconstituted the cells with either WT human IR B isoform (WT-IR) or a mutant IR in which leucine-973 has been changed to phenylalanine (L973F-IR) as well as by creation of a knockin mouse model with an INSR L973F substitution

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Normally, the filled vials are placed on trays of the machine, then frozen at -40-60C to convert the product into ice