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Ewing Weight Loss Clinic has helped thousands of patients in Reno and the surrounding areasincluding Sparks, Carson City, and Lake Tahoetake control of their weight with safe and medically supervised programs

14 PCR amplification was performed in a final volume of 25 l of the reaction mixture containing 1.5 U of Taq DNA polymerase (Sibenzyme), about 1 g DNA of each patient's DNA, 0.25 M each primer, 250 M of dNTPs, 2 mM of MgCl 2 , 1 PCR buffer of the following composition: 67 mM Tris-HCl pH 8.8, 16.6 mM (NH 4 ) 2 SO 4 and 0.01% Tween-20

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Ferrer-Suay S, Alonso-Iglesias E, Tortajada-Girbs M, Carrasco-Luna J, Codoer-Franch P