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Some chelators, such as D-penicillamine, also cause direct neurotoxicity, likely by crossing the blood-brain barrier and disrupting copper-dependent central nervous system enzymes, impairing energy metabolism, and increasing oxidative stress [245, 246]

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An isoelectric point (pI) of 5.8, and net charges of +2.8 and 14.7 at pH 5.5 and pH 7.4, respectively, were calculated for the albumin variants (Supplementary Table 3)