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of these, 3060% can be annotated to a metabolite name at MS1 mass-match level (confidence level 3), 1530% confirmed by MS2 spectral matching against HMDB or MassBank (confidence level 2), and 515% confirmed by MS2 + retention time match against authentic standards (confidence level 1)

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AHAs and BHAs: Alpha hydroxy acids like glycolic acid and beta hydroxy acids like salicylic acid create low-pH environments that can destabilize copper peptide structures