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In aqueous media, incorporation of the isotopic label from bulk solvent into flavan-3,3,4-triol ( 4 ) was anticipated to lead to distinct m / z and 18 O incorporation patterns of the observed adducts and flavonoid-typical C-ring cleavage fragments

Immunoblotting To profile protein lipoylation and lysine glutarylation, 0.5 10 6 HeLa cells or 1 10 6 T cells were lysed in SDS loading buffer (2% (wt/vol) SDS, 50 mM Tris (pH 7.4), 150 mM NaCl, 1 mM dithiothreitol, 10% (vol/vol) glycerol and 0.2% (vol/vol) benzonase nuclease (Sigma-Aldrich, E1014)) and heated at 90 C for 5 min

Vitamin B12 B12 deserves its own mention because its deficiency is widespread and its consequences significant including fatigue, brain fog, and peripheral nerve symptoms