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However, GLUT-4 mRNA expression was lower in L6 myotubes compared with the control group (Fig

Then liposomes labeled with the lipophilic fluorescent dye DiD were added to the apical chamber of the Transwell plates and incubated at 37 C for 2 h, after which the bEnd.3 cells were fixed with 4% PFA at room temperature for 1 h and stained with DAPI at room temperature for 5 min

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Abrupt switches between morning and evening dosing disrupt circadian synchronization and may produce 35 days of reduced effectiveness while hormonal rhythms re-entrain to the new schedule