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After 30 min of puromycin treatment, cells were lysed in RIPA Lysis and Extraction Buffer (Cat# 89901) that included Halt Protease Inhibitor Cocktail (Cat# 78430) and Halt Phosphatase Inhibitor Cocktail (Cat# 78420) from Thermo Fisher Scientific (Waltham, MA, USA)

TCs were found in air samples collected during the drying and milling process on farms, in the ventilation systems of private houses and office buildings and on the walls of houses with high humidity [89-90]