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Beyond this narrow indication, methylene blue is widely employed off-label for intraoperative sentinel lymph node mapping, ifosfamide-induced neurotoxicity, and refractory vasoplegic (distributive) shock, with additional investigational uses in neuroprotection (Alzheimer disease, stroke, traumatic brain injury), psychiatric disorders (as a monoamine oxidase inhibitor), and even historical antimalarial therapy

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Elevated IL-6 and TNF- concentrations in CMS exposed mice demonstrate stress-induced inflammatory signaling contribution toward anxiety-like behaviors (Tyagi et al., 2025)

The peptide operates through multiple interconnected pathways rather than a single target, which accounts for its diverse benefits