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Heatmap showing fold change in metabolite levels (log 2 ) of the indicated cell lines relative to the average of those in SLC25A39 knockout cells complemented with SLC25A39 cDNA (middle)

Subsequent efforts to identify the enzyme responsible for cGAMP production led to the characterization of cyclic GMP-AMP synthase (cGAS), a cytosolic DNA sensor that directly binds double-stranded DNA and catalyzes cGAMP synthesis upon activation (Sun et al., 2013)