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Hansen JM, Go YM, Jones DP
Early mutagenesis studies of the Arabidopsis GS identified a putative glycine-rich loop as important for activity (Wang and Oliver, 1997a,b), which was identified in later structural studies as forming part of the active site (Galant et al., Arabidopsis GS uses a random Ter-reactant mechanism with binding of either ATP or -glutamylcysteine preferred first and addition of glycine last (Jez and Cahoon, Arabidopsis GS and for the eukaryotic homologs (Herrera et al., 2+ to ATP and orienting the -phosphate group in the active site
The cycle structure exists for a practical reason: continuous stimulation of the same receptor pathways can lead to reduced sensitivity over time, and a rest period allows the system to reset before the next cycle produces full effect
Because it not only neutralizes free radicals directly but also regenerates other antioxidants like vitamin C and E
