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What to monitor Visible changes: Skin texture and tone Fine line depth Hair density and quality Wound healing speed Subjective factors: Energy levels Sleep quality General sense of wellbeing Side effects: Injection site reactions Skin flushing Fatigue (especially early in use) Photograph yourself in consistent lighting at baseline and every 4 weeks

18, 32043213.10.1093/emboj/18.12.3204 53 RawlinsM

Nrf2 staining and translocation For visualization and quantification of endogenous Nrf2 translocation into the nucleus, glass 96-well plates were coated with fibronectin (1 g/ml) (Sigma) and poly-l-ornithine (40 g/ml) over night