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These doses sit well within the range tested in animal safety studies and far below levels where any toxicity was observed

After an overnight incubation with the enzyme, 30 l of a blank (1 PBS), a glucose standard (twofold serial dilutions from 0.01 to 0.16 mg/ml glucose) and duplicates of the samples were pipetted into a 96-well plate and the assay was then performed according to the GAGO-20 kit protocol as described above