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Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

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We consider vein access, appointment timing, and whether you are comfortable with IV therapy

This is particularly relevant for GLP-1 users, whose dietary patterns may be reducing the natural substrates that support these bacteria