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Following injury, the aggregation of neutrophils significantly contributes to activating and maintaining early inflammatory responses, which are necessary for the initial stage of the healing process and introduction of further tissue damage (62)

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Supernatant was transferred to 1.5 mL microcentrifuge tube after which the remining pellet was resuspended in 400 L 20 C 40:40:20 Acetonitrile (ACN): MeOH: H2O extraction solvent and incubated on ice for 5 minutes

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