After 48 h, 160 l of expression medium (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-galactose (Panreac, A1131), 67 mM potassium phosphate monobasic buffer pH 6.0 (Thermo Scientific, 424200250), 25 g l 1 ethanol (Scharlau, ET00062500), 2 mM copper sulfate (Merck, C7631) and 25 g ml 1 chloramphenicol (Merck, C0378)) was added to each well
When paired with vitamin C, absorption improves significantly, making results faster and more stable
Patients already avoiding steroids due to hepatic or renal concerns should be evaluated carefully, as the combined metabolic load of an aggressive peptide protocol warrants individual assessment
Diamine Oxidase (DAO) This enzyme is your first line of defense