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For euthanasia and terminal sampling, systemic anesthesia was achieved through the administration of an intraperitoneal cocktail comprising acetylpromazine (2.5 mg/kg) and ketamine (100 mg/kg)

For any target anchor dose D (mg) and anchor mass M in the vial (GHK-Cu = 50 mg, BPC-157 = 10 mg): V water = V draw M D Worked example 3 mg GHK-Cu, 20-unit (0.2 mL) draw: V water = 0.2 mL 50 mg3 mg = 3.33 mL BAC water The GLOW Calculator above solves this for any anchor and draw volume