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However, HFD-feeding has been shown to induce alpha-cell hyperplasia prior to the expansion of beta-cell mass in mice (17, 100), and similarly, studies performed in normoglycemic non-human primates demonstrate that alpha-cell mass was more significantly augmented in response to the increased duration and severity of obesity than that of beta-cells (101)

Kidney injury molecule 1 assay The renal concentration of kidney injury molecule 1 (KIM-1) was determined in kidney homogenates using enzyme-linked immunosorbent assay (ELISA) kit obtained from Abcam (Catalogue No

doi: 10.1097/CRD.0000000000000513, 111

149,150 Once in the nucleus, Nrf2 activates a suite of antioxidant response element-containing genes