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Within these frameworks, the distance between specific molecules serves as a proxy for chemical or functional similarity

Transwell assay The chemotactic motility of HUVECs was determined using transwell migration chambers (Corning) with 6.5 mm diameter polycarbonate filters (8 m pore size), as described previously.28 In brief, the bottom chambers were filled with 750 mL of RPMI 1640 medium containing all supplements