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Blood samples were collected from the tail vein, and glucose levels were measured by the glucose oxidase method with OneTouch Ultra glucometer (LifeScan, Malvern, USA)

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To investigate what underlines changes in Ca 2+ transients, we assessed levels and phosphorylation state of three major proteins that control the time constant and amplitude of Ca 2+ transient: Na + Ca 2+ exchanger (NCX1), sarco-endoplasmic reticulum Ca 2+ -ATPase (SERCa2), and phospholamban (PLN) (Figure 5C)