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In our system, Cr GPDH further converts Gro3P to glycerol and Pi, which prevents Gro3P accumulation and allows uninterrupted NAD + recycling (because mGPDH is coenzyme Q linked, diminished electron flow via ETC decreases the activity of endogenous Gro3P shuttle and we previously observed elevated levels of both DHAP and Gro3P under conditions of NADH-reductive stress) ( Figure 1a ) 15

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The reasons for an increased circulating Hcy are variable and range from imbalanced diet, poor in leafy greens (shortage of folates) or, opposite, entirely vegetarian (shortage of B12), to a bad fit between the diet and the inherited genetic variants of the concerned metabolic enzymes, in many cases circulating in double digit prevalence, which may generate an increased demand (relative shortage) of several dietary substrates [14]