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The Downstream Risks That Follow Severe Gastrointestinal Effects Severe vomiting and diarrhoea are not only unpleasant

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100 g wet weight of cells were harvested by centrifugation (10 min at 1000 g ), washed twice in Tris-HCl buffer (10 mM Tris-HCl, pH 7.5), and chilled on ice